Summary

  • Hydrolysed collagen was found to inhibit inflammation in fibroblasts and keratinocytes, alongside improving collagen synthesis of Pro Collagen-1a by fibroblasts while also increasing the numbers of both fibroblasts and keratinocytes indicating that collagen can reduce inflammation, improve the immune response and increase skin cells that improve all skin functions.*


Abstract

Background: 

A study aimed to investigate if collagen peptides can enhance fibroblast and keratinocyte proliferation and activation while reducing inflammation caused by lipopolysaccharide (LPS).

Methods: 

The study used human skin fibroblasts and keratinocytes and tested three different doses of collagen peptides along with LPS. The parameters for evaluation were cell proliferation, pro-collagen-1? expression by fibroblasts, inducible nitric oxide synthase (iNOS) expression, and cytokine secretion by both cell types.

Results: 

The results showed that all doses of collagen supplementation led to increased proliferation of fibroblasts and keratinocytes, while only the highest dose induced increased expression of pro-collagen-1? by fibroblasts. Collagen supplementation also suppressed LPS-induced iNOS expression and reduced the secretion of inflammatory cytokines while increasing the expression of growth factors TGF-? and VEGF.

Conclusions: 

In summary, collagen peptides were found to enhance the proliferation of fibroblasts and keratinocytes while reducing inflammation caused by LPS, thus indicating their potential in improving skin health. The study highlighted the importance of dose-dependent effects of collagen and suggested that higher doses may have a more significant impact. Overall, these findings support the use of collagen in various skin-related products to enhance their effectiveness.

Glossary

*Fibroblasts – Main type of skin cells involved in in tissue repair, fibrogenesis and the initiation of an immune response.

*Keratinocytes – A type of skin cell that creates keratin and initiates an immune response (e.g. creating interleukins in response to microbes)

*Collagen – Main extracellular matrix protein of skin. Reduces skin stiffening and increases skin humidity and elasticity.

Source: Hydrolysed Collagen Induces an Anti-Inflammatory Response That Induces Proliferation of Skin Fibroblast and Keratinocytes